Overlapping PCR for Bidirectional PCR Amplification of Specific Alleles: A Rapid One-Tube Method for Simultaneously Differentiating Homozygotes and Heterozygotes
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چکیده
منابع مشابه
Single-tube method for determination of F508del genotype in the CFTR gene using bidirectional PCR amplification of specific alleles.
Cystic fibrosis is the most common severe autosomal recessive disorder in Caucasians, with a frequency of 1 in 2000 live births and a carrier frequency of approximately 5% (1). It is a multi-system disorder that leads to chronic pulmonary and exocrine pancreatic disease, associated with elevated sweat electrolytes and male infertility due to bilateral absence of the vas deferens (2–4). Mutation...
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BACKGROUND Common methods for identification of DNA sequence variants use gel electrophoresis or column separation after PCR. METHODS We developed a method for sequence variant analysis requiring only PCR and amplicon melting analysis. One of the PCR primers was fluorescently labeled. After PCR, the melting transition of the amplicon was monitored by high-resolution melting analysis. Differen...
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A rapid, inexpensive, simple, and accurate multiplex polymerase chain reaction (PCR) was developed in a single tube for identification of Mycobacterium tuberculosis. Assessment of sensitivity and specificity of simple PCR was performed with 116 strains of M. tuberculosis complex (MTC) and 144 strains of nontuberculous mycobacteria (NTM) compared with the biochemical method. Specific amplificati...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1997
ISSN: 1088-9051,1549-5469
DOI: 10.1101/gr.7.4.389